https://wiki.uni-konstanz.de/ccp4/api.php?action=feedcontributions&feedformat=atom&user=DocandreasCCP4 wiki - User contributions [en]2026-04-17T06:44:11ZUser contributionsMediaWiki 1.43.8https://wiki.uni-konstanz.de/ccp4/index.php?title=Crystal_mounting&diff=1054Crystal mounting2008-08-01T11:53:16Z<p>Docandreas: New page: == Nomenclature == The crystal sits in the loop, the loop atop a pin, which is attached, usually with superglue, to a base (most frequently magnetic). Hampton calls the whole assembly Cry...</p>
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<div>== Nomenclature ==<br />
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The crystal sits in the loop, the loop atop a pin, which is attached, usually with superglue, to a base (most frequently magnetic). Hampton calls the whole assembly CrystalCap. Caps protected by a vial can be stored in liquid nitrogen.<br />
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== Potential problems after mounting the cap onto the goniometer head ==<br />
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* ice crystals at the bottom of the base - These will slowly thaw and move the loop away from the cryostream ==> use only dry caps for freezing crystals<br />
* loose pins in the base => superglue pin into base and let set before freezing<br />
* magnets too weak (base too heavy)<br />
* magnet too strong, leading to whiplash effect and catapulting the crystal from the loop (when freezing crystals directly on the beamline, not when mounting prefrozen crystals)<br />
* incorrectly fastened goniometer, magnet etc.</div>Docandreashttps://wiki.uni-konstanz.de/ccp4/index.php?title=Crystals&diff=1053Crystals2008-08-01T11:41:45Z<p>Docandreas: /* Crystal Growth */</p>
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<div>== Crystal Characterization ==<br />
* [[Birefringence]]<br />
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== Crystal Growth ==<br />
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* [[Modifying the protein to crystallize better]]<br />
* [[Crystallization screens and methods]]<br />
* [[I have crystals, but are they salt?]]<br />
* [[Conditions prone to salt crystallization]]<br />
* [[Biophysical methods]] for evaluation of crystallization tendency<br />
* [[Robots for crystallization setup]]<br />
* [[Visualization of crystal growth]]<br />
* [[Crystal mounting]]<br />
* [[Robots for crystal mounting]]<br />
* [[Crystal growth: Tips and Tricks]]<br />
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== Data collection ==<br />
* [[X-ray generators and detectors]] (experiences) <br />
* [[Synchrotrons]]<br />
* [[Cryo]]<br />
* [[Strategies]]<br />
* [[Data quality]]<br />
* [[Radiation damage]]<br />
* [[Data collection: Tips and Tricks]]</div>Docandreas