Solving a structure: Tips and Tricks

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One possible way of improving "weak density" is to improve the model as much as you can: make sure you modeled all alternative conformations, all solvent, etc.

  • B-factor sharpening may help, although keep in mind that it will enhance the noise too.
  • Other options: kick maps, omit kick maps, b-factor sharpened kick maps... available from Phenix
  • You can try GrowDensity method (Acta Cryst. (1997). D53, 540-543) which is available as phenix.grow_density. This is still under (slow) development, so if you decide to go this route than the Phenix developers can help with the details.