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Cryo: Difference between revisions
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* Fill the liquid propane into vials. For short time storage and crystal handling put the vials in a flat liquid nitrogen bath. If propane becomes solid after a while just remove the vial from the liquid nitrogen and put it on the desk; let it stand for a while until it is liquid again and put it pack into the nitrogen bath. | * Fill the liquid propane into vials. For short time storage and crystal handling put the vials in a flat liquid nitrogen bath. If propane becomes solid after a while just remove the vial from the liquid nitrogen and put it on the desk; let it stand for a while until it is liquid again and put it pack into the nitrogen bath. | ||
* Plunge the crystals just into the vials and wait until the propane is solid. | * Plunge the crystals just into the vials and wait until the propane is solid. | ||
== Salts as precipitants ... == | |||
Some salts may also serve as cryoprotectants: malonate, formate, ammonium sulfate (at >3.5 M), lithium sulfate, and perhaps others. Anything with a flat solubility vs temperature is a good candidate. Mixing of different cryos can often have a superior protective effect to single-component cryos of the same total concentration (the "confusion principle"), so there are a lot of combinations to try. | |||
== See also == | == See also == | ||
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* http://idb.exst.jaxa.jp/db_data/protein/search-e.php? Cryoprotectant database | * http://idb.exst.jaxa.jp/db_data/protein/search-e.php? Cryoprotectant database | ||
* or see concentrations given in: McFerrin and Snell, J.Appl.Cryst (2002) 35, 538 and Mitchell and Garman, J.Appl.Cryst. (1996) 29, 584 | * or see concentrations given in: McFerrin and Snell, J.Appl.Cryst (2002) 35, 538 and Mitchell and Garman, J.Appl.Cryst. (1996) 29, 584 | ||
* faster freezing: a simple means (blowing away the gas layer) is described by Warkentin et al. (2006). J. Appl. Crystallogr. 39, 805. | |||