Modifying the protein to crystallize better: Difference between revisions
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== [[Lysine Methylation]]== | == [[Lysine Methylation]]== | ||
This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum] | This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum]. The idea is to change the charge properties post-purification, modify the solubility of the protein (hence the phase diagram) and get crystals! Its a quick and easy thing to try especially for proteins that appear to be too soluble! | ||
Revision as of 12:51, 10 February 2008
Lysine Methylation
This protocol is an NKI local adaptation based on the article by Grimes and co-workers[1]. The idea is to change the charge properties post-purification, modify the solubility of the protein (hence the phase diagram) and get crystals! Its a quick and easy thing to try especially for proteins that appear to be too soluble!