Crystals: Difference between revisions
Jump to navigation
Jump to search
(solvent content) |
|||
Line 1: | Line 1: | ||
== Crystal Characterization == | == Crystal Characterization == | ||
* [[Birefringence]] | * [[Birefringence]] | ||
* [[solvent content]] | |||
== Crystal Imperfections == | == Crystal Imperfections == | ||
Line 23: | Line 24: | ||
* [[BMCD|Biomolecular crystallization database]] | * [[BMCD|Biomolecular crystallization database]] | ||
* Prediction of protein crystallizability: [http://ffas.burnham.org/XtalPred-cgi/xtal.pl XtalPred] | * Prediction of protein crystallizability: [http://ffas.burnham.org/XtalPred-cgi/xtal.pl XtalPred] | ||
* crystallization of soluble proteins (see also [[Improving crystal quality]]) | |||
* crystallization of membrane proteins (see also [[Improving crystal quality#Improving diffraction quality of membrane protein crystals]]) | |||
== Crystal handling == | == Crystal handling == | ||
Line 41: | Line 44: | ||
== References == | == References == | ||
# Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372. | # Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372. | ||
# Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153. | # Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153. |
Revision as of 08:23, 13 March 2012
Crystal Characterization
Crystal Imperfections
Crystal Growth
- Modifying the protein to crystallize better
- Crystallization screens and methods
- I have crystals, but are they salt?
- Conditions prone to salt crystallization
- Biophysical methods for evaluation of crystallization tendency
- Improving crystal quality
- Robots for crystallization setup
- Visualization of crystal growth
- Crystal mounting
- Robots for crystal mounting
- Robots for crystallization monitoring
- Crystal growth: Tips and Tricks
- Crystal growth: Protein-DNA complexes
- Biomolecular crystallization database
- Prediction of protein crystallizability: XtalPred
- crystallization of soluble proteins (see also Improving crystal quality)
- crystallization of membrane proteins (see also Improving crystal quality#Improving diffraction quality of membrane protein crystals)
Crystal handling
Data collection
- X-ray generators and detectors (experiences)
- Synchrotrons
- Strategies
- Data quality
- Radiation damage
- Comparison of home source and synchrotron properties
- Data collection: Tips and Tricks
References
- Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372.
- Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153.
- Methods and Results in Crystallization of Membrane Proteins Iwata S (Ed.), San Diego, CA: International University Line. (2003)
- Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules. Doublie S (Ed.), Totowa, NJ: Humana Press. (2007)
- Structural Biology of Membrane Proteins. Grisshammer R, & Buchanan SK (Eds.), Cambridge, UK: Royal Society of Chemistry. (2006)
- Macromolecular Crystallisation and Crystal Perfection. N E Chayen, J R Helliwell and E H Snell OUP 2010 [1]