Topics

Expression and Purification

• Expression systems
• Purification
• Cleavage: wanted and unwanted
• DNA and DNA-binding proteins
• Expression and Purification: Tips and Tricks

Crystals

Crystal growth and manipulation

• Crystallization screens and methods
• Robots for crystallization setup
• Visualization of crystal growth
• Robots for crystal mounting
• Crystal growth: Tips and Tricks

Data collection

• Strategies
• Data quality

Crystallography

Theory

• Anomalous scattering
• Friedel's law
• centric and acentric reflections
• choice of origin
• ways to index a diffraction pattern
• symmetry and space groups
• R-factors
• direct methods
• experimental phasing
• molecular replacement

Programs

by package (large packages first)

• CCP4
• CNS
• PHENIX
• SHELX C/D/E (homepage at [1])
• XDS homepage and XDSwiki
• ...

by task

• Data reduction
• Molecular replacement (MR)
• Experimental phasing (SIR/MIR and SAD/MAD)
• Refinement

Bioinformatics and computers

• Servers and programs for sequence analysis (e.g. alignment)
• Servers and programs for structure analysis
• Prediction of structure from sequence: servers and programs
• Visualization: programs, graphics cards and 3D
• Computer hardware