Soaking with heavy atoms: Difference between revisions
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* Bart Hazes' [http://eagle.mmid.med.ualberta.ca/tutorials/HA/ collection of properties of heavy metals] | * Bart Hazes' [http://eagle.mmid.med.ualberta.ca/tutorials/HA/ collection of properties of heavy metals] - if unavailable try [http://web.archive.org/web/20071028111005/http://eagle.mmid.med.ualberta.ca/tutorials/HA/ here] | ||
* "Towards a rational approach for heavy-atom derivative screening in protein crystallography" by Agniswamy et al, [http://dx.doi.org/10.1107/S0907444907068849 Acta Cryst D 64, 354] | * "Towards a rational approach for heavy-atom derivative screening in protein crystallography" by Agniswamy et al, [http://dx.doi.org/10.1107/S0907444907068849 Acta Cryst D 64, 354] | ||
* Michael Sawaya's [http://www.doe-mbi.ucla.edu/~sawaya/tutorials/Phasing/references.html Preparing a Useful Derivative] | * Michael Sawaya's [http://www.doe-mbi.ucla.edu/~sawaya/tutorials/Phasing/references.html Preparing a Useful Derivative] | ||
* Artem Evdokimov's [http://www.xtals.org/pdfs/iodination.pdf Iodination of protein crystals for fun and profit] | * Artem Evdokimov's [http://www.xtals.org/pdfs/iodination.pdf Iodination of protein crystals for fun and profit] | ||
* Sugahara et al's Heavy-atom Database System ([http://hatodas.harima.riken.go.jp/ HATODAS]) - a WWW-based tool designed to assist the heavy-atom derivatization of proteins. | |||
* http://www.sbg.bio.ic.ac.uk/had/ - good table of HA and pH ranges | |||
* http://sis.niaid.nih.gov/cgi-bin/heavyatom_reactivity.cgi - Heavy Atom Reactivity Table ([http://sis.niaid.nih.gov/pub_pdf/Agniswamy-et-al-2008.pdf original paper]) | |||
Links to a variety of HA databases: http://www.shapirolab.org/lab-links/had.html | |||
References: [http://dx.doi.org/10.1107/S0907444903020584 Lott JS et al. (2003) Acta Cryst D Biol Crystallogr, Dec (Pt 12): 2242-6. Making the most of two crystals: structural analysis of a conserved hypothetical protein using native gel screening and SAD phasing.] | |||
Latest revision as of 14:26, 17 July 2009
- Bart Hazes' collection of properties of heavy metals - if unavailable try here
- "Towards a rational approach for heavy-atom derivative screening in protein crystallography" by Agniswamy et al, Acta Cryst D 64, 354
- Michael Sawaya's Preparing a Useful Derivative
- Artem Evdokimov's Iodination of protein crystals for fun and profit
- Sugahara et al's Heavy-atom Database System (HATODAS) - a WWW-based tool designed to assist the heavy-atom derivatization of proteins.
- http://www.sbg.bio.ic.ac.uk/had/ - good table of HA and pH ranges
- http://sis.niaid.nih.gov/cgi-bin/heavyatom_reactivity.cgi - Heavy Atom Reactivity Table (original paper)
Links to a variety of HA databases: http://www.shapirolab.org/lab-links/had.html