Course or Conference 4: Difference between revisions

From CCP4 wiki
Jump to navigation Jump to search
(New page: The goal of the course is to present methods of macromolecular crystallization and give hands-on experience with them. Participants may bring their own proteins. The course is divided in...)
 
(Removing all content from page)
 
(2 intermediate revisions by 2 users not shown)
Line 1: Line 1:


The goal of the course is to present methods of macromolecular crystallization and give hands-on experience with them.  Participants may bring their own proteins. The course is divided into 50% lectures and 50% laboratory sessions.  Limit: 20 participants.
*Topics to be covered by lectures and laboratory sessions include:
*Conventional techniques and their modifications.
*High-throughput crystallization screening.
*The path to crystals.  The phase diagram and the kinetics of equilibration.
*Optimization: Exploiting protein formulation, cocktail formulation, and time to equilibration.
*Using a microfluidic device to determine protein precipitation boundaries.
*A pro-active strategy for crystallization.
*Oils as tools for crystallization.
*How to choose your method of crystallization.
*Seeding as an optimization tool.
*Interpretation of the crystallization drop phenomenon:  recognizing what you see.
*Does it matter what screen that you use?
*Biophysical tools for crystallization:  mass spectroscopy, dynamic light scattering, Thermofluor.
*Crystal clear communication: the role of LIMS in a crystallization laboratory.
*Cryoprotection.
[[http://xray.bmc.uu.se/~terese/course/course.htm Official sourse page]]

Latest revision as of 17:08, 18 October 2008

Retrieved from "https://wiki.uni-konstanz.de/ccp4/index.php?title=Course_or_Conference_4&oldid=1144"