Expression systems: Difference between revisions
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Algae | Algae | ||
Chloroplasts | Chloroplasts | ||
''Nicotiana tabacum'' | |||
= Try Rosetta cells = | |||
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest". | If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest". | ||
--[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET) | --[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET) | ||
Revision as of 09:35, 11 February 2008
Bacteria
Escherichia coli Bacillus subtilis Bacillus megaterium Pseudomonas fluorescens
Protozoa
Leishmania tarentolae
Fungi
Pichia pastoris Saccharomyces cerevisiae Schizosaccharomyces pombe Hansenula polymorpha Filamentous fungi
Insect cells
Baculovirus-mediated expression in "Spodoptera frugiperda" and "Trichoplusia ni" cells
Mammalian cells
Chinese hamster ovary cultured cells
Plant cells
Algae Chloroplasts Nicotiana tabacum
Try Rosetta cells
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".
--Artem 02:43, 11 February 2008 (CET)