Crystals: Difference between revisions
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* crystallization of soluble proteins (see also [[Improving crystal quality]]) | * crystallization of soluble proteins (see also [[Improving crystal quality]]) | ||
* crystallization of membrane proteins (see also [[Improving crystal quality#Improving diffraction quality of membrane protein crystals]]) | * crystallization of membrane proteins (see also [[Improving crystal quality#Improving diffraction quality of membrane protein crystals]]) | ||
* relative humidity calculations of salt/PEG solutions - can also be used to calculate equivalent/matching osmotic pressure: http://go.esrf.eu/RH | |||
== Crystal handling == | == Crystal handling == | ||
Revision as of 13:58, 27 July 2014
Crystal Characterization
Crystal Imperfections
Crystal Growth
- Modifying the protein to crystallize better
- Crystallization screens and methods
- I have crystals, but are they salt?
- Conditions prone to salt crystallization
- Biophysical methods for evaluation of crystallization tendency
- Improving crystal quality
- Robots for crystallization setup
- Visualization of crystal growth
- Crystal mounting
- Robots for crystal mounting
- Robots for crystallization monitoring
- Crystal growth: Tips and Tricks
- Crystal growth: Protein-DNA complexes
- Biomolecular crystallization database
- Prediction of protein crystallizability: XtalPred
- crystallization of soluble proteins (see also Improving crystal quality)
- crystallization of membrane proteins (see also Improving crystal quality#Improving diffraction quality of membrane protein crystals)
- relative humidity calculations of salt/PEG solutions - can also be used to calculate equivalent/matching osmotic pressure: http://go.esrf.eu/RH
Crystal handling
Data collection
- X-ray generators and detectors (experiences)
- Synchrotrons
- Strategies
- Data quality
- Radiation damage
- Comparison of home source and synchrotron properties
- Data collection: Tips and Tricks
- Storage of diffraction data
References
- Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372.
- Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153.
- Methods and Results in Crystallization of Membrane Proteins Iwata S (Ed.), San Diego, CA: International University Line. (2003)
- Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules. Doublie S (Ed.), Totowa, NJ: Humana Press. (2007)
- Structural Biology of Membrane Proteins. Grisshammer R, & Buchanan SK (Eds.), Cambridge, UK: Royal Society of Chemistry. (2006)
- Macromolecular Crystallisation and Crystal Perfection. N E Chayen, J R Helliwell and E H Snell OUP 2010 [1]