Conditions prone to salt crystallization: Difference between revisions
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<td>yes </td> | <td>yes </td> | ||
<td>Crystals reproduced with proteins and with buffer alone at least twice --[[User:Emeric.gueneau|Emeric.gueneau]] 18:21, 16 July 2008 (CEST)</td> | <td>Crystals reproduced with proteins and with buffer alone at least twice --[[User:Emeric.gueneau|Emeric.gueneau]] 18:21, 16 July 2008 (CEST)</td> | ||
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<td>Qiagen/Nextal Anions #5 and #6</td> | |||
<td>0.3 M and 0.6 M NaF in NaOAc pH 4.6 </td> | |||
<td>10mM Tris pH7.5, 150mM NaCl </td> | |||
<td>yes </td> | |||
<td>Gives hexagon shaped crystals every time- see line one - beware with NaF </td> | |||
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Revision as of 14:33, 23 July 2008
For some background information please see I have crystals, but are they salt?.
This list shall not include instances when significant drying of the drop occurred. This list should not be taken to mean that the given conditions always produce salt crystals, but they apparently sometimes do.
| Screen and # | Condition | Protein buffer | Confirmed by diffraction | Signature and comment |
| Hampton PEG/ion #1 | 0.2M NaF, 20% PEG3350, pH7.1 | 10mM Tris pH7.5, 150mM NaCl | yes | I've seen this condition produce salt crystals at least twice.--Ed 16:18, 23 May 2008 (CEST) Ditto - NaF has caused me trouble many times - buyer beware! DaveB 11:01, 24 May 2008 (CEST) |
| Qiagen
Plate 3 MembFac & Natrix equivalent |
1M Ammonium Sulfate, 0.1M Sodium Acetate | 25mM NaPO4 pH8, 150mM NaCl | yes | Crystals reproduced with proteins and with buffer alone at least twice --Emeric.gueneau 18:21, 16 July 2008 (CEST) |
| Qiagen/Nextal Anions #5 and #6 | 0.3 M and 0.6 M NaF in NaOAc pH 4.6 | 10mM Tris pH7.5, 150mM NaCl | yes | Gives hexagon shaped crystals every time- see line one - beware with NaF |
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