Thiols and disulfides

Expression of proteins containing disulfides (editing in progress, April 23, 2008)

Expression of proteins containing disulfides in the native state can make a lot of trouble when expressed using standard vectors and strains for cytoplasmic expression.

Several possibilities are availbale for successful expression 1. Expression targetetd to the periplasm 2. Expression in yeast and secretion into the medium 3. Expression in strains with defects in maintaining the low redxpotential of the cytplasm

Analysis of free thiols

Analysis of disulfides