10 February 2008
- 13:2613:26, 10 February 2008 diff hist +838 N Lysine Methylation New page: This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Re...
- 13:2413:24, 10 February 2008 diff hist +24 N Modifying the protein to crystallize better New page: * Lysine Methylation
- 13:2313:23, 10 February 2008 diff hist +50 m Topics →Crystal growth and manipulation
- 13:1513:15, 10 February 2008 diff hist +312 m Main Page a lits of chapter under "topics" is now added in front page, linked to the chapters
- 12:5812:58, 10 February 2008 diff hist +2,639 N Spheroplasts Plates New page: ==Day 0: Overnight growth of 96 selected clones== *Starting from rearraying list, perform an overnight culture of 96 clones in 1 ml of LB with antibiotics to taste. *To avoid any mistakes... current
- 12:5712:57, 10 February 2008 diff hist +2,062 N Spheroplasts Falcon New page: ==Spheroplasts lysis starting from Falcon tube cultures== *Prepare 50 ml falcon tubes with 12.5ml of medium plus antibiotics. *Transfer 125 uL of a saturated preculture. *Put falcons in i... current
- 12:5712:57, 10 February 2008 diff hist +221 N Expression screening New page: ==Expression Testing== *Cell lysis with Spheroplasts in 96-well Plates - From Darren Hart - Grenoble *[[Spheroplasts Falcon|Cell lysis with Spheroplasts in Falcon ... current
- 12:5312:53, 10 February 2008 diff hist +27 Topics →Expression and Purification
- 12:5212:52, 10 February 2008 diff hist 0 Topics →by package
- 12:5212:52, 10 February 2008 diff hist +15 Topics →by package