Crystals

Crystal Characterization

• Birefringence

Crystal Imperfections

• Diffuse Scattering
• Incommensurate Lattices

Crystal Growth

• Modifying the protein to crystallize better
• Crystallization screens and methods
• I have crystals, but are they salt?
• Conditions prone to salt crystallization
• Biophysical methods for evaluation of crystallization tendency
• Robots for crystallization setup
• Visualization of crystal growth
• Crystal mounting
• Robots for crystal mounting
• Robots for crystallization monitoring
• Crystal growth: Tips and Tricks

Crystal handling

• Sticky crystals

Data collection

• X-ray generators and detectors (experiences)
• Synchrotrons
• Cryo
• Strategies
• Data quality
• Radiation damage
• Data collection: Tips and Tricks

References

• crystallization of soluble proteins

• crystallization of membrane proteins

1. Wiener MC (2004) A pedestrian guide to membrane protein crystallization. Methods 34(3), 364-372.
2. Loll PJ (2003) Membrane protein structural biology: the high throughput challenge. J. Struct. Biol. 142(1), 144-153.
3. Methods and Results in Crystallization of Membrane Proteins Iwata S (Ed.), San Diego, CA: International University Line. (2003)
4. Macromolecular Crystallography Protocols, Volume 1: Preparation and Crystallization of Macromolecules. Doublie S (Ed.), Totowa, NJ: Humana Press. (2007)
5. Structural Biology of Membrane Proteins. Grisshammer R, & Buchanan SK (Eds.), Cambridge, UK: Royal Society of Chemistry. (2006)