Expression systems

Bacteria

  Escherichia coli
  Bacillus subtilis
  Bacillus megaterium
  Pseudomonas fluorescens

Protozoa

  Leishmania tarentolae

Fungi

  Pichia pastoris
  Saccharomyces cerevisiae
  Schizosaccharomyces pombe
  Hansenula polymorpha
  Filamentous fungi

Insect cells

  Baculovirus-mediated expression in "Spodoptera frugiperda" and "Trichoplusia ni" cells

Mammalian cells

  Chinese hamster ovary cultured cells

Plant cells

  Algae
  Chloroplasts

Try Rosetta cells

If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest".

  Nicotiana tabacum

--Artem 02:43, 11 February 2008 (CET)