Topics

Expression and Purification

• Expression systems
• Purification
• Cleavage: wanted and unwanted
• DNA and DNA-binding proteins
• Expression and Purification: Tips and Tricks

Crystals

Crystal growth and manipulation

• Crystallization screens and methods
• Robots for crystallization setup
• Visualization of crystal growth
• Robots for crystal mounting
• Crystal growth: Tips and Tricks

Data collection

• Synchrotron beamlines
• Cryo
• Strategies
• Data quality

Crystallography

Theory

• Basics of Crystallography
• Phase problem, and Phasing
• R-factors
• References and links

Programs

by package

(large packages first)

• CCP4
• CNS
• PHENIX
• SHELX C/D/E/L (homepage at [1])
• moleman, lsqman, ... from Uppsala Software Factory
• XDS homepage and XDSwiki
• HKL homepage
• ...

by task

• Data reduction
• Molecular replacement (MR)
• Experimental phasing (SIR/MIR and SAD/MAD)
• Model building and validation
• Refinement

Bioinformatics and computers

• Servers and programs for sequence analysis (e.g. alignment)
• Servers and programs for protein structure analysis
• Servers for ligand topologies/parameters
• Prediction of structure from sequence: servers and programs
• Visualization: programs, graphics cards and 3D
• Computer hardware
• Operating systems and Linux distributions

FAQ and troubleshooting

• protein is not expressed
• crystals don't grow
• phases too bad
• model does not refine