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| == Biophysical Characterization of Macromolecular Complexes, NKI Amsterdam, May 21-23, 2008 ==
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| Structural Biology of macromolecular complexes needs to always be complemented
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| by biophysical studies to understand the details of the molecular interactions
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| that determine function. In this practical “hands-on” course we will
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| examine in detail:
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| *Surface Plasmon Resonance (SPR). Determination of kinetic parameters of interaction (kon, koff) and affinity (KD) from time-resolution experiments; screening of ligand arrays for protein binding.
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| *Isothermal Titration Calorimetry (ITC). Determination of thermodynamic parameters of interactions (ΔG,ΔH) and (KD) from ligand titration experiments.
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| *Multi-Angle Laser Light Scattering (MALLS)Determination of molecular weight of complexes concurrent with size exclusion chromatography.
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| [[http://xtal.nki.nl/BioCourse_08.pdf PDF poster]]
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