1,330
edits
Expression of SeMet labeled proteins: Difference between revisions
no edit summary
(re-format and add non-bacterial) |
No edit summary |
||
| Line 1: | Line 1: | ||
= bacterial expression | = bacterial expression = | ||
| Line 108: | Line 108: | ||
= expression | = expression in other systems = | ||
* mammalian cells: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf mentions several mammalian cell lines, including CHO, HEK239, and COS. Apparently, Lec8 have been used, too. | * mammalian cells: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2242577/pdf/2008.pdf mentions several mammalian cell lines, including CHO, HEK239, and COS. Apparently, Lec8 have been used, too. | ||
* Yeast | * Yeast | ||
* stable S2 insect cells | * stable S2 insect cells | ||
* baculovirus expression system | * baculovirus expression system | ||
* CHO LecR cells, early reference (1994) from Wayne Hendrickson's lab (PMID: 7922031) | |||
* probably the easiest labelling method for HEK293 cells can be found in PMID: 17001101. | |||