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Structural Biology of macromolecular complexes needs to always be complemented | |||
by biophysical studies to understand the details of the molecular interactions | |||
that determine function. In this practical “hands-on” course we will | |||
examine in detail: | |||
*Surface Plasmon Resonance (SPR). Determination of kinetic parameters of interaction (kon, koff) and affinity (KD) from time-resolution experiments; screening of ligand arrays for protein binding. | |||
*Isothermal Titration Calorimetry (ITC). Determination of thermodynamic parameters of interactions (ΔG,ΔH) and (KD) from ligand titration experiments. | |||
*Multi-Angle Laser Light Scattering (MALLS)Determination of molecular weight of complexes concurrent with size exclusion chromatography. | |||
[[http://xtal.nki.nl/BioCourse_08.pdf PDF poster]] | |||