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Thiols and disulfides: Difference between revisions
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Several possibilities are availbale for successful expression | Several possibilities are availbale for successful expression | ||
* Expression targeted to the periplasm | * Expression targeted to the periplasm. Several vectors for E.coli are available, e.g. containing the signal sequence of OmpA or for expression as MalE fusion. | ||
* Expression in yeast and secretion into the medium | * Expression in yeast and secretion into the medium. | ||
* Expression in strains with defects in maintaining the low | Here are also several systems available. Check out the current catalogues of molecular biology supply. | ||
* Expression in strains with defects in maintaining the low redoxpotential of the cytoplasm. | |||