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Crystal growth: Tips and Tricks: Difference between revisions
→I tried Hampton Screen I & II with my protein and have no crystals. What can I do?
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# Set your screens at different temperature. 25 and 4 degrees are the most popular options. If you completely lost hope for your original screens, just transfer them to the cold room. Protein solubility will change and you may get crystals. | # Set your screens at different temperature. 25 and 4 degrees are the most popular options. If you completely lost hope for your original screens, just transfer them to the cold room. Protein solubility will change and you may get crystals. | ||
# Add a purification step. Purity can be critical to protein crystallization and the purity judged from SDS-PAGE or even gel filtration can be misleading. | # Add a purification step. Purity can be critical to protein crystallization and the purity judged from SDS-PAGE or even gel filtration can be misleading. | ||
# There are other screens. While it is proably true that when proteins do crystalize, they in many cases produce hits from Hampton screens I or II, we are dealing here with exception to that rule. Lists of screens are available from manufacturer websites: | # There are other screens. While it is proably true that when proteins do crystalize, they in many cases produce hits from Hampton screens I or II, we are dealing here with exception to that rule. Lists of screens are available from manufacturer websites: | ||
#* [http://www.hamptonresearch.com/ Hampton Research] | #* [http://www.hamptonresearch.com/ Hampton Research] | ||
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#* [http://www.jenabioscience.com/cms/en/1/browse/632_screens.html Jena Bioscience] | #* [http://www.jenabioscience.com/cms/en/1/browse/632_screens.html Jena Bioscience] | ||
#* [[Crystallization screens and methods | Other screens ]] | #* [[Crystallization screens and methods | Other screens ]] | ||
# [[Modifying_the_protein_to_crystallize_better | Modify your protein. ]] | |||
==Improving crystals== | ==Improving crystals== | ||