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The Milan protocol ...: Difference between revisions
New page: =SLIC Sub-cloning using T4 DNA polymerase treated insert= * Digest 3 μg vector with two restriction enzymes. Gel-purify the vector and isolate the DNA using Qiagen extraction kit. Quanti...
(New page: =SLIC Sub-cloning using T4 DNA polymerase treated insert= * Digest 3 μg vector with two restriction enzymes. Gel-purify the vector and isolate the DNA using Qiagen extraction kit. Quanti...) |
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