Crystal growth: Protein-DNA complexes: Difference between revisions

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(New page: Tips suggested by the CCP4 community as the best way to crystallize a protein/DNA complex 1. One of the biggest variables is to screen different lengths of DNA 2. Good to try both blunt...)
 
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7. Other useful tips?
7. Other useful tips?


Protein DNA crystals can be very fragile so good idea to screen with cryoprotectant in the screen to save manipulations
* Protein DNA crystals can be very fragile so good idea to screen with cryoprotectant in the screen to save manipulations
Test crystals with a gel to see if DNA is present
* Test crystals with a gel to see if DNA is present
Use of SAXS to get molecular envelop of complex
* Use of SAXS to get molecular envelop of complex
* general guidelines to prepare protein/DNA complexes suitable for crystallization trials: [http://dx.doi.org/10.1007/978-1-59745-209-0_11 Hollis T (2007) Crystallization of protein-DNA complexes. Methods Mol. Biol. 363: 225–237]
* design and composition of a specialized screen for protein/DNA complexes: [http://dx.doi.org/10.1107/S1744309112025316 Pryor EE Jr, Wozniak DJ & Hollis T (2012) Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein-DNA crystals. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68: 985–993]




Several references below:
== Some references: ==


Schultz, S. C., Shields, G. C. & Steitz, T. A. (1990).
Schultz, S. C., Shields, G. C. & Steitz, T. A. (1990).
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