Data collection: Tips and Tricks: Difference between revisions
Jump to navigation
Jump to search
(New page: == cryo == * PEG3350 seems to be little too large for freezing, particularly in presence of high salt concentration. However, PEG2000 is good enough as a cryo protectant. * Try oils ...) |
(No difference)
|
Revision as of 11:14, 15 February 2009
cryo
- PEG3350 seems to be little too large for freezing, particularly in presence of high salt concentration. However, PEG2000 is good enough as a cryo protectant.
- Try oils
- Try stepwise increase from low concentrations of cryoprotectants to the required concentration, over several minutes/hours/days.
- The larger the crystal, the longer should be the incubation in each step.
- Small crystals often do better in terms of mosaicity increase than big crystals.