Data collection: Tips and Tricks
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- PEG3350 seems to be little too large for freezing, particularly in presence of high salt concentration. However, PEG2000 is good enough as a cryo protectant.
- Try oils
- Try stepwise increase from low concentrations of cryoprotectants to the required concentration, over several minutes/hours/days.
- The larger the crystal, the longer should be the incubation in each step.
- Small crystals often do better in terms of mosaicity increase than big crystals.