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Detergent concentration: Difference between revisions
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This article is about how to measure | This article is about how to measure detergent concentration after concentrating a membrane protein sample. | ||
1. The simplest way to control the detergent concentration is to use a | 1. The simplest way to control the detergent concentration is to use a | ||
higher cut-off concentrator if | higher cut-off concentrator if your protein plus detergent micelle is large | ||
enough. Michael Matho: “a 50kDa cutoff withheld a lot of detergent during | enough. Michael Matho: “a 50kDa cutoff withheld a lot of detergent during | ||
concentration process and consequently your final concentration might | concentration process and consequently your final concentration might | ||
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increase sensitivity, speedvac a volume too large to spot on the plate, | increase sensitivity, speedvac a volume too large to spot on the plate, | ||
dissolve the residue in MeOH.” | dissolve the residue in MeOH.” | ||
A strategy for identification and quantification of detergents frequently | A strategy for identification and quantification of detergents frequently | ||
used in the purification of membrane proteins. Laura R. Eriks, June A. | used in the purification of membrane proteins. Laura R. Eriks, June A. | ||