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(Changed to a more of a chpater format to encourage people to post more details !?!?) |
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= | =Mainstream systems= | ||
==''Escherichia coli''== | |||
===Rosetta cells === | |||
=Protozoa= | If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest". | ||
==Fungi== | |||
===''Pichia pastoris''=== | |||
==Insect cells== | |||
===Baculovirus-mediated expression in ''Spodoptera frugiperd'' and ''Trichoplusia ni'' cells=== | |||
==Mammalian cells== | |||
===Chinese hamster ovary (CHO) cultured cells=== | |||
===Human Embryonic Kidney (HEK) 293 cultured cells === | |||
*[http://www.ncbi.nlm.nih.gov/pubmed/17001101?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Transient transfection]. | |||
*[http://www.invitrogen.com/content.cfm?pageid=3375 Flp-In for stable cell lines ]. | |||
=Other systems= | |||
==Bacteria== | |||
===''Bacillus subtilis''=== | |||
===''Bacillus megaterium''=== | |||
===''Pseudomonas fluorescens''=== | |||
==Protozoa== | |||
===''Leishmania tarentolae''=== | |||
=Fungi= | ==Fungi== | ||
= | ===''Saccharomyces cerevisiae''=== | ||
===''Schizosaccharomyces pombe''=== | |||
===''Hansenula polymorpha''=== | |||
===''Filamentous fungi''=== | |||
=Plant cells= | =Plant cells= | ||
= | ==Algae== | ||
Chloroplasts | |||
''Nicotiana tabacum'' | |||
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