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Phase problem: Difference between revisions
→Experimental phasing
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A recent introduction into the principles of phase calculation with the help of experiments (SIR/MIR/SIRAS/MIRAS/SAD/MAD) can be e.g. found in Taylor, G. (2003) The phase problem. Acta Cryst D59, 1881-1890 [http://journals.iucr.org/d/issues/2003/11/00/ba5050/index.html]. Requirement for applicability of [[experimental phasing]] is [[isomorphism]] between heavy-atom derivative and native (i.e. the macromolecule itself, and the crystal's cell should be unchanged), specific binding of heavy atom, and enough heavy-atom incorporation into the crystal. | A recent introduction into the principles of phase calculation with the help of experiments (SIR/MIR/SIRAS/MIRAS/SAD/MAD) can be e.g. found in Taylor, G. (2003) The phase problem. Acta Cryst D59, 1881-1890 [http://journals.iucr.org/d/issues/2003/11/00/ba5050/index.html]. Requirement for applicability of [[experimental phasing]] is [[isomorphism]] between heavy-atom derivative and native (i.e. the macromolecule itself, and the crystal's cell should be unchanged), specific binding of heavy atom, and enough heavy-atom incorporation into the crystal. | ||
A vey good recent paper is A. J. McCoy and R. J. Read (2010) Experimental phasing: best practice and pitfalls. Acta Cryst D66, 458-469 (open access at [http://dx.doi.org/10.1107/S0907444910006335]) | |||
== [[molecular replacement|Molecular Replacement]] (MR) == | == [[molecular replacement|Molecular Replacement]] (MR) == | ||