1,328
edits
Crystal growth: Protein-DNA complexes: Difference between revisions
Jump to navigation
Jump to search
Crystal growth: Protein-DNA complexes (edit)
Revision as of 10:54, 9 September 2015
, 9 September 2015no edit summary
(New page: Tips suggested by the CCP4 community as the best way to crystallize a protein/DNA complex 1. One of the biggest variables is to screen different lengths of DNA 2. Good to try both blunt...) |
No edit summary |
||
| Line 16: | Line 16: | ||
7. Other useful tips? | 7. Other useful tips? | ||
Protein DNA crystals can be very fragile so good idea to screen with cryoprotectant in the screen to save manipulations | * Protein DNA crystals can be very fragile so good idea to screen with cryoprotectant in the screen to save manipulations | ||
Test crystals with a gel to see if DNA is present | * Test crystals with a gel to see if DNA is present | ||
Use of SAXS to get molecular envelop of complex | * Use of SAXS to get molecular envelop of complex | ||
* general guidelines to prepare protein/DNA complexes suitable for crystallization trials: [http://dx.doi.org/10.1007/978-1-59745-209-0_11 Hollis T (2007) Crystallization of protein-DNA complexes. Methods Mol. Biol. 363: 225–237] | |||
* design and composition of a specialized screen for protein/DNA complexes: [http://dx.doi.org/10.1107/S1744309112025316 Pryor EE Jr, Wozniak DJ & Hollis T (2012) Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein-DNA crystals. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 68: 985–993] | |||
== Some references: == | |||
Schultz, S. C., Shields, G. C. & Steitz, T. A. (1990). | Schultz, S. C., Shields, G. C. & Steitz, T. A. (1990). | ||