Crystallization screens and methods: Difference between revisions

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Because it is not generally possible to rationally predict crystallization conditions, screens have been developed which sample a wide range of crystallization space in order to identify preliminary crystallization condititions.
Many such screens are commercially available and fall into two categories:
1. Sparse-matrix screens, which contain conditions that have previously proved successful.
2. Grid screens, in which specific crystallization conditions are sampled in a systematic way.
3. Imcomplete factorial screens, in which individual components of crystallization conditions are varied in a systematic way.
==The PACT screen==
==The PACT screen==
The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space[http://scripts.iucr.org/cgi-bin/paper?cy5025]. The PACT screen is available from Molecular Dimensions[http://www.moleculardimensions.com/us/merchant.ihtml?pid=2403&step=4] and Qiagen [http://www1.qiagen.com/Products/Protein/Crystallization/CoreScreensForInitialInvestigations/PACTSuite.aspx?ShowInfo=1]
The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space[http://scripts.iucr.org/cgi-bin/paper?cy5025]. The PACT screen is available from Molecular Dimensions[http://www.moleculardimensions.com/us/merchant.ihtml?pid=2403&step=4] and Qiagen [http://www1.qiagen.com/Products/Protein/Crystallization/CoreScreensForInitialInvestigations/PACTSuite.aspx?ShowInfo=1]
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