The Paris protocol ...

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This work is in progress, based on pET21a inserting between NdeI and NotI sites.

The protocol being used is essentially as in Elledge, 2007, with RecA.

See supplementary protcol #2 file - via CNRS Direct Nature URL from

Li M.Z. & Elledge S. J. Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nature Methods 4, 251 - 256 (2007) doi:10.1038/nmeth1010