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== Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism == | == Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism == | ||
The structure of AcrB was solved by molecular replacement, followed by many cycles of refinement and model building. The raw data are available at ftp://turn5.biologie.uni-konstanz.de/pub/datasets/ . | The structure of AcrB in spacegroup C2 was solved by molecular replacement (using the symmetric structure as a model), followed by many cycles of refinement and model building. The raw data are available at ftp://turn5.biologie.uni-konstanz.de/pub/datasets/ . | ||
Reference: Seeger, M.A., Schiefner, A., Eicher, T., Verrey, F., Diederichs, K., Pos, K.M. (2006) Structural Asymmetry of AcrB Trimer Suggests a Peristaltic Pump Mechanism. ''Science'' '''313''': 1295-1298 | Reference: Seeger, M.A., Schiefner, A., Eicher, T., Verrey, F., Diederichs, K., Pos, K.M. (2006) Structural Asymmetry of AcrB Trimer Suggests a Peristaltic Pump Mechanism. ''Science'' '''313''': 1295-1298 | ||
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The goal of this XDSwiki project is to run different versions of XDS (and other data reduction programs if anybody would contribute the data reductions!), and to compare internal R-factor statistics against R-factors of the refined model. | The goal of this XDSwiki project is to run different versions of XDS (and other data reduction programs if anybody would contribute the data reductions!), and to compare internal R-factor statistics against R-factors of the refined model. | ||
More to come | More to come! | ||
Revision as of 17:45, 6 June 2008
Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism
The structure of AcrB in spacegroup C2 was solved by molecular replacement (using the symmetric structure as a model), followed by many cycles of refinement and model building. The raw data are available at ftp://turn5.biologie.uni-konstanz.de/pub/datasets/ .
Reference: Seeger, M.A., Schiefner, A., Eicher, T., Verrey, F., Diederichs, K., Pos, K.M. (2006) Structural Asymmetry of AcrB Trimer Suggests a Peristaltic Pump Mechanism. Science 313: 1295-1298
The goal of this XDSwiki project is to run different versions of XDS (and other data reduction programs if anybody would contribute the data reductions!), and to compare internal R-factor statistics against R-factors of the refined model.
More to come!