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1RQW: Difference between revisions
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→Thaumatin Br soak: structure solution from either 2-wavelength MAD, or from single wavelength SAD
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==Thaumatin Br soak: structure solution from either 2-wavelength MAD, or from single wavelength SAD== | ==Thaumatin Br soak: structure solution from either 2-wavelength MAD, or from single wavelength SAD== | ||
Thaumatin is a sweet-tasting protein of 207 residues that crystallizes in P4<sub>1</sub>2<sub>1</sub>2 with a monomer in the asymmetric unit. | |||
Prior to flash cooling in liquid nitrogen at 100 K the crystals were soaked for a few seconds in a solution containing 1 M sodium bromide and 25% (v/v) glycerol. | Prior to flash cooling in liquid nitrogen at 100 K the crystals were soaked for a few seconds in a solution containing 1 M sodium bromide and 25% (v/v) glycerol. | ||