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Crystal growth: Tips and Tricks: Difference between revisions
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→Reducing the mosaicity: links and rearrangement vs cryo articles
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==Improving crystals== | ==Improving crystals== | ||
===Reducing the mosaicity=== | ===Reducing the mosaicity=== | ||
Things to try | |||
Things to try concerning crystallization - see also [[Improving crystal quality]]. | |||
For cryo question see [[cryo]] and [[Data collection: Tips and Tricks]]. | |||
* change precipitant, pH, temperature; try additives | |||
* try co-crystallization with glycerol (2%, 5% or 10%). | |||
* incubate your crystal longer in your cryo/stabilization solution. | |||
* optimize the cryo conditions | |||
* anneal (freeze/thaw) the crystal - '''DISCLAIMER''': your mosaicity can go up as well as down with annealing | |||
* the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis | |||
* look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??) | |||
==References== | ==References== | ||