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Crystallization screens and methods: Difference between revisions
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Crystallization screens and methods (edit)
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(New page: * The PACT screen The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple ...) |
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==The PACT screen== | |||
The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space[http://scripts.iucr.org/cgi-bin/paper?cy5025]. The PACT screen is available from Molecular Dimensions[http://www.moleculardimensions.com/us/merchant.ihtml?pid=2403&step=4] and Qiagen [http://www1.qiagen.com/Products/Protein/Crystallization/CoreScreensForInitialInvestigations/PACTSuite.aspx?ShowInfo=1] | The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space[http://scripts.iucr.org/cgi-bin/paper?cy5025]. The PACT screen is available from Molecular Dimensions[http://www.moleculardimensions.com/us/merchant.ihtml?pid=2403&step=4] and Qiagen [http://www1.qiagen.com/Products/Protein/Crystallization/CoreScreensForInitialInvestigations/PACTSuite.aspx?ShowInfo=1] | ||
under license, and by Jena Biosciences[http://www.jenabioscience.com/cms/en/1/catalog/1184_jbscreen_pact.html]. | under license, and by Jena Biosciences[http://www.jenabioscience.com/cms/en/1/catalog/1184_jbscreen_pact.html]. | ||
The exact formulations and all information for how to make the PACT screen are available as supplementary material of the original article by Newman et al[http://scripts.iucr.org/cgi-bin/sendsupfiles?cy5025&file=cy5025sup1.pdf&mime=application/pdf] and are available under Open Access policy. | The exact formulations and all information for how to make the PACT screen are available as supplementary material of the original article by Newman et al[http://scripts.iucr.org/cgi-bin/sendsupfiles?cy5025&file=cy5025sup1.pdf&mime=application/pdf] and are available under Open Access policy. | ||