Crystallization screens and methods: Difference between revisions

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(New page: * The PACT screen The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple ...)
 
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* [[The PACT screen]]
==The PACT screen==
The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space[http://scripts.iucr.org/cgi-bin/paper?cy5025]. The PACT screen is available from Molecular Dimensions[http://www.moleculardimensions.com/us/merchant.ihtml?pid=2403&step=4] and Qiagen [http://www1.qiagen.com/Products/Protein/Crystallization/CoreScreensForInitialInvestigations/PACTSuite.aspx?ShowInfo=1]
The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple the components of each condition and to provide information about the protein, even in the absence of crystals, rather than cover a wide crystallization space[http://scripts.iucr.org/cgi-bin/paper?cy5025]. The PACT screen is available from Molecular Dimensions[http://www.moleculardimensions.com/us/merchant.ihtml?pid=2403&step=4] and Qiagen [http://www1.qiagen.com/Products/Protein/Crystallization/CoreScreensForInitialInvestigations/PACTSuite.aspx?ShowInfo=1]
under license, and by Jena Biosciences[http://www.jenabioscience.com/cms/en/1/catalog/1184_jbscreen_pact.html].
under license, and by Jena Biosciences[http://www.jenabioscience.com/cms/en/1/catalog/1184_jbscreen_pact.html].


The exact formulations and all information for how to make the PACT screen are available as supplementary material of the original article by Newman et al[http://scripts.iucr.org/cgi-bin/sendsupfiles?cy5025&file=cy5025sup1.pdf&mime=application/pdf] and are available under Open Access policy.
The exact formulations and all information for how to make the PACT screen are available as supplementary material of the original article by Newman et al[http://scripts.iucr.org/cgi-bin/sendsupfiles?cy5025&file=cy5025sup1.pdf&mime=application/pdf] and are available under Open Access policy.
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