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Expression systems: Difference between revisions
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Algae | Algae | ||
Chloroplasts | Chloroplasts | ||
''Nicotiana tabacum'' | |||
= Try Rosetta cells = | |||
If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest". | If you are trying to express non-bacterial protein, it is quite likely that your gene of interest contains rare codons and thus E.Coli can't express it well. Rosetta and Rosetta 2 cells (available from Novagen) are designed to overcome this problem. For human proteins, expression levels may sometimes change from "I need to enrich the protein batch on affinity column before I can see protein on the gel" to "80% of the protein in the lysed cells is my protein of interest". | ||
--[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET) | --[[User:Zelig ff|Artem]] 02:43, 11 February 2008 (CET) | ||