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Intensity statistics: Difference between revisions
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The data I am working on has a strong translation vector (this can be found out e.g. using [http://www.ccp4.ac.uk/dist/html/sfcheck.html sfcheck]). | The data I am working on has a strong translation vector (this can be found out e.g. using [http://www.ccp4.ac.uk/dist/html/sfcheck.html sfcheck]). | ||
On the cumulative intensity distribution plot, the | On the cumulative intensity distribution plot, the theoretical and observed curves do not overlap. I did "detect_twinning" from CNS, and there is the | ||
result: | result: | ||
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=== Answer (slightly edited): === | === Answer (slightly edited): === | ||
A normal data set has a unimodal intensity distribution with a predictable shape (for formulas and plots, see [[Centric and acentric reflections]]). When there is [[twinning]] the distribution remains unimodal but becomes sharper and this is picked up in the twinning analysis. When there is pseudo-translational symmetry, as you indicate you have, then the intensity distribution becomes bimodal with one set of reflections systematically strengthened and another systematically weakened. This makes the whole distribution broader, just the opposite of what twinning does, and therefore shows up as "negative twinning" in the analysis. | A normal data set has a unimodal intensity distribution with a predictable shape (for formulas and plots, see [[Centric and acentric reflections]]). When there is [[twinning]] the distribution remains unimodal but becomes sharper and this is picked up in the twinning analysis. When there is [[pseudo-translation|pseudo-translational]] symmetry, as you indicate you have, then the intensity distribution becomes bimodal with one set of reflections systematically strengthened and another systematically weakened. This makes the whole distribution broader, just the opposite of what twinning does, and therefore shows up as "negative twinning" in the analysis. | ||
== Mean intensity as a function of resolution == | == Mean intensity as a function of resolution == | ||
Please see [[Wilson plot]]. | Please see [[Wilson plot]]. | ||