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Modifying the protein to crystallize better: Difference between revisions
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Modifying the protein to crystallize better (edit)
Revision as of 16:26, 8 November 2019
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== Introduction== | == Introduction== | ||
There are a number of methods which can be used to modify the crystallization behavior of proteins. | There are a number of methods which can be used to modify the crystallization behavior of proteins. | ||
=== Genetic/Mutagenesis Approach=== | === Genetic/Mutagenesis Approach=== | ||
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There is a webserver, called SER (Surface Entropy Reduction Prediction Server), which aims to predict sites that are most suitable for mutation designed to enhance crystallizability: | There is a webserver, called SER (Surface Entropy Reduction Prediction Server), which aims to predict sites that are most suitable for mutation designed to enhance crystallizability: | ||
https://services.mbi.ucla.edu/SER/intro.php | |||
=== Chemical Modification=== | === Chemical Modification=== | ||
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A recent study (Walter et al. [2006] "Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization" Structure 14:1617–1622) demonstrated that, out of 10 non-crystallizable proteins, | A recent study (Walter et al. [2006] "Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization" Structure 14:1617–1622) demonstrated that, out of 10 non-crystallizable proteins, | ||
4 proteins became crystallisable after reductive methylation. | 4 proteins became crystallisable after reductive methylation. | ||
Here is a protocol for [[Lysine Methylation]]. | Here is a protocol for [[Lysine Methylation]]. | ||