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Expression and Purification: Tips and Tricks: Difference between revisions
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Expression and Purification: Tips and Tricks (edit)
Revision as of 19:07, 4 March 2008
, 4 March 2008→Protein precipitates?
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To increase solubility, follow Golovanov et al. 2004. In short, | To increase solubility, follow Golovanov et al. 2004. In short, | ||
* buy L-Arg and L-Glu | * buy L-Arg and L-Glu (i.e. don't use salts) | ||
* put L- | * put L-Arg powder into water, start dissolving it, then add L-Glu powder, stir; add water to adjust volume as required | ||
* max. solubility is 1 M. The pH of a 1M stock solution will be around 6.4 or close to neutral | * max. solubility is 1 M. The pH of a 1M stock solution will be around 6.4 or close to neutral | ||
* add stock solution (50mM) to your protein | * add stock solution (50mM) to your protein | ||