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Modifying the protein to crystallize better: Difference between revisions
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Modifying the protein to crystallize better (edit)
Revision as of 02:16, 9 March 2008
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== [[Introduction]]== | == [[Introduction]]== | ||
There are a number of methods which can be used to modify the crystallization behavior of proteins. | There are a number of methods which can be used to modify the crystallization behavior of proteins. | ||
These fall into two classes: | These fall into two classes: | ||
1. Genetic (Mutagenesis) Approach | 1. Genetic (Mutagenesis) Approach | ||
2. Chemical Modification | 2. Chemical Modification | ||
== [[Genetic/Mutagenesis Approach]]== | |||
== [[1. Genetic/Mutagenesis Approach]]== | |||
This method, also called 'surface entropy-reduction mutagenesis', involves mutation of sidechains on the surface of proteins, in order to reduce the entropic cost of forming ordered intermolecular crystal contacts and thus enhance the crystallizability of proteins. | This method, also called 'surface entropy-reduction mutagenesis', involves mutation of sidechains on the surface of proteins, in order to reduce the entropic cost of forming ordered intermolecular crystal contacts and thus enhance the crystallizability of proteins. | ||
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== [[Chemical Modification]]== | == [[2. Chemical Modification]]== | ||
Another method for enhancing the crystallizability of proteins involves chemical modification of specific sidechains. | Another method for enhancing the crystallizability of proteins involves chemical modification of specific sidechains. | ||
Several methods are available: | Several methods are available: | ||