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Modifying the protein to crystallize better: Difference between revisions
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Modifying the protein to crystallize better (edit)
Revision as of 18:36, 9 March 2008
, 9 March 2008→[[2. Chemical Modification]]
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A recent study (Walter et al. [2006] "Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization" Structure 14:1617–1622) demonstrated that, out of 10 non-crystallizable proteins, | A recent study (Walter et al. [2006] "Lysine Methylation as a Routine Rescue Strategy for Protein Crystallization" Structure 14:1617–1622) demonstrated that, out of 10 non-crystallizable proteins, | ||
4 proteins became crystallisable after reductive methylation. | 4 proteins became crystallisable after reductive methylation. | ||
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Here is a protocol for [[Lysine Methylation]]. | |||
2.2. Modification of cysteine residues | |||
2.2. Modification of | |||
This method involves the carboxymethylation -- under reducing conditions -- of single cysteine residues. This effectively neutralizes the cysteine residues (some of which are chemically reactive), increases the overall solubility of the protein and can help prevent aggregation and denaturation problems. | This method involves the carboxymethylation -- under reducing conditions -- of single cysteine residues. This effectively neutralizes the cysteine residues (some of which are chemically reactive), increases the overall solubility of the protein and can help prevent aggregation and denaturation problems. | ||