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Lysine Methylation: Difference between revisions
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m (corrected spelling mistake) |
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Remove precipitated protein (up to 50%) by centrifugation | Remove precipitated protein (up to 50%) by centrifugation | ||
Concentrate and put protein on Gel Filtration column in a Tris | Concentrate and put protein on Gel Filtration column in a Tris containing buffer, eg: | ||
*20mM Tris (pH7.5) | *20mM Tris (pH7.5) | ||
*200mM NaCl | *200mM NaCl | ||
*1mM DTT | *1mM DTT | ||