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Crystallization screens and methods: Difference between revisions
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→Desiccation of an existing screen which shows no sign of crystallization or precipitation
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''This reports a discussion on CCP4BB started on April 7, 2008 under the topic "which concentrated salt has lowest vapour pressure?".'' | ''This reports a discussion on CCP4BB started on April 7, 2008 under the topic "which concentrated salt has lowest vapour pressure?".'' | ||
If, after setup of a crystallization experiment and waiting long enough, the droplets stay clear, the question arises whether one can modify this experiment to learn something. | If, after setup of a crystallization experiment and waiting long enough, the droplets stay clear, the question arises whether one can modify this experiment to still learn something. | ||
Thus the idea is that instead of setting up a new experiment, one simply modifies the reservoir in an attempt to make the protein crystallize (or precipitate), with the goal of bracketing the range of precipitant concentration. | Thus the idea is that instead of setting up a new experiment (and losing the protein), one simply modifies the reservoir in an attempt to make the protein crystallize (or precipitate), with the goal of bracketing the range of precipitant concentration. | ||
This idea has been explored in a number of publications: | This idea has been explored in a number of publications: | ||
* H. Schreuder et al. published a method to measure the dehydration effect of precipitants (J. Appl. Cryst. (1988). 21, 426-429.). This publication has tables of the chemical potential of water in solutions of MPD, NaCl, potassium tratrate and PEG 6K. | * H. Schreuder et al. published a method to measure the dehydration effect of precipitants (J. Appl. Cryst. (1988). 21, 426-429.). This publication has tables of the chemical potential of water in solutions of MPD, NaCl, potassium tratrate and PEG 6K. | ||