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Thiols and disulfides: Difference between revisions
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(New page: Expression of proteins containing disulfides Analysis of free thiols Analysis of disulfides) |
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Expression of proteins containing disulfides | Expression of proteins containing disulfides (editing in progress, April 23, 2008) | ||
Expression of proteins containing disulfides in the native state can make a lot of trouble when expressed using standard vectors and strains for cytoplasmic expression. | |||
Several possibilities are availbale for successful expression | |||
1. Expression targetetd to the periplasm | |||
2. Expression in yeast and secretion into the medium | |||
3. Expression in strains with defects in maintaining the low redxpotential of the cytplasm | |||
Analysis of free thiols | Analysis of free thiols | ||
Analysis of disulfides | Analysis of disulfides | ||