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Thiols and disulfides: Difference between revisions
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Several possibilities are availbale for successful expression | Several possibilities are availbale for successful expression | ||
* Expression targeted to the periplasm. Several vectors for E.coli are available, e.g. containing the signal sequence of OmpA or for expression as MalE fusion. | * Expression targeted to the periplasm. Several vectors for ''E.coli'' are available, e.g. containing the signal sequence of OmpA or for expression as MalE fusion. | ||
* Expression in yeast and secretion into the medium.Here are also several systems available. Check out the current catalogues of molecular biology supply. | * Expression in yeast and secretion into the medium.Here are also several systems available. Check out the current catalogues of molecular biology supply. | ||
* Expression in E.coli strains with defects in maintaining the low redox potential of the cytoplasm. Strains carrying mutations in thioredoxin reductase (trxB-, ADA494) or glutathione reductase (gor-) or both (Origami strain) allow disulfide formation in the cytoplasm. Even expression of multiple Ig domains is possible in the Origami strain. | * Expression in ''E.coli'' strains with defects in maintaining the low redox potential of the cytoplasm. Strains carrying mutations in thioredoxin reductase (trxB-, ADA494) or glutathione reductase (gor-) or both (Origami strain) allow disulfide formation in the cytoplasm. Even expression of multiple Ig domains is possible in the Origami strain. | ||