Thiols and disulfides: Difference between revisions
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(New page: Expression of proteins containing disulfides Analysis of free thiols Analysis of disulfides) |
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Expression of proteins containing disulfides | Expression of proteins containing disulfides (editing in progress, April 23, 2008) | ||
Expression of proteins containing disulfides in the native state can make a lot of trouble when expressed using standard vectors and strains for cytoplasmic expression. | |||
Several possibilities are availbale for successful expression | |||
1. Expression targetetd to the periplasm | |||
2. Expression in yeast and secretion into the medium | |||
3. Expression in strains with defects in maintaining the low redxpotential of the cytplasm | |||
Analysis of free thiols | Analysis of free thiols | ||
Analysis of disulfides | Analysis of disulfides | ||
Revision as of 13:37, 24 April 2008
Expression of proteins containing disulfides (editing in progress, April 23, 2008)
Expression of proteins containing disulfides in the native state can make a lot of trouble when expressed using standard vectors and strains for cytoplasmic expression.
Several possibilities are availbale for successful expression 1. Expression targetetd to the periplasm 2. Expression in yeast and secretion into the medium 3. Expression in strains with defects in maintaining the low redxpotential of the cytplasm
Analysis of free thiols
Analysis of disulfides