User contributions for Tassos

18 February 2008

• 14:1614:16, 18 February 2008 diff hist +98‎ Current events ‎ new course
• 14:1314:13, 18 February 2008 diff hist +91‎ m Current events ‎ chronological order!
• 14:1114:11, 18 February 2008 diff hist +28‎ N Courses and Conferences ‎ Courses and Conferences moved to Current events: fits there a bit better really - consensus with Kay current
• 14:1114:11, 18 February 2008 diff hist 0‎ m Current events ‎ Courses and Conferences moved to Current events: fits there a bit better really - consensus with Kay
• 13:5813:58, 18 February 2008 diff hist +207‎ N User talk:Kay ‎ New page: Kay - should I maybe move the courses and conferences to the Current Events space ? I would also be inclined to display it with headers and no separate pages for each. Give me the green ...
• 13:5413:54, 18 February 2008 diff hist +615‎ N Course or Conference 3 ‎ New page: The current trend in structural biology is to study macromolecular assemblies of ever-increasing size and complexity. This EMBO practical course aims to teach the strategic use of biochemi...
• 13:5313:53, 18 February 2008 diff hist +109‎ Current events ‎No edit summary
• 13:4313:43, 18 February 2008 diff hist −3‎ m Main Page ‎No edit summary
• 13:4313:43, 18 February 2008 diff hist −17‎ m Main Page ‎No edit summary
• 13:4113:41, 18 February 2008 diff hist +774‎ Course or Conference 2 ‎No edit summary
• 13:3913:39, 18 February 2008 diff hist +118‎ Current events ‎No edit summary
• 11:5211:52, 18 February 2008 diff hist 0‎ m Main Page ‎ Most recent first !
• 11:3511:35, 18 February 2008 diff hist +2‎ m Visualization: graphics cards and 3D ‎ →‎graphics cards and drivers
• 11:3511:35, 18 February 2008 diff hist +1,918‎ Visualization: graphics cards and 3D ‎ Added dual desktop support for Nvidia drivers - might be superseded with new driver versions!
• 11:2811:28, 18 February 2008 diff hist +35‎ m Visualization: graphics cards and 3D ‎ introduced ,code> tags to the "code"

15 February 2008

• 13:2113:21, 15 February 2008 diff hist 0‎ m Topics ‎ →‎Expression and Purification
• 13:2013:20, 15 February 2008 diff hist +45‎ m User talk:Tassos ‎No edit summary current
• 13:1913:19, 15 February 2008 diff hist +2‎ m SLIC cloning ‎No edit summary
• 13:1413:14, 15 February 2008 diff hist +120‎ N SLIC cloning ‎ New page: The Heidelberg protocol ... The Milan protocol ... The Paris protocol ... A contribution from 3D-Repertoire
• 13:1313:13, 15 February 2008 diff hist +19‎ m Topics ‎ →‎Expression and Purification

12 February 2008

• 17:3417:34, 12 February 2008 diff hist 0‎ Expression systems ‎ →‎Mammalian cells current
• 17:3317:33, 12 February 2008 diff hist +320‎ m Expression systems ‎ →‎Human Embryonic Kidney (HEK) 293 cultured cells
• 17:3017:30, 12 February 2008 diff hist +719‎ Expression systems ‎ →‎Human Embryonic Kidney (HEK) 293 cultured cells
• 17:2117:21, 12 February 2008 diff hist +224‎ N User:Tassos ‎ New page: '''Anastassis (Tassos) Perrakis ''' Principal Investigator / Staff Member Netherlands Cancer Institute, Dept. H2 1066 CX Amsterdam, The Netherlands Tel: +31 20 512 1951 Fax: +31 20 512... current
• 17:1917:19, 12 February 2008 diff hist +137‎ N User talk:Rogerdodd ‎ New page: - Hi Artem: why do you use this format that put sthings in this dot-line box ? Could you please explain ? i think I am missing something.
• 17:0317:03, 12 February 2008 diff hist +24‎ Expression systems ‎ Changed to a more of a chpater format to encourage people to post more details !?!?
• 15:4915:49, 12 February 2008 diff hist +334‎ Expression systems ‎ →‎Mammalian cells
• 15:4015:40, 12 February 2008 diff hist +16‎ m Coot ‎ Moved the coot to the side of the contents - again aesthetics ...?
• 15:3615:36, 12 February 2008 diff hist −9‎ m Main Page ‎ a detail for coot - maybe it reads better now

11 February 2008

• 18:1118:11, 11 February 2008 diff hist +207‎ N Servers and programs for sequence analysis ‎ added my favorite tools -biased
• 18:1018:10, 11 February 2008 diff hist +182‎ N Structure based sequence alignment ‎ a few servers to start with ...
• 18:0918:09, 11 February 2008 diff hist +24‎ m Topics ‎ →‎Bioinformatics

10 February 2008

• 12:5912:59, 10 February 2008 diff hist +292‎ N ARP/wARP ‎ New page: ARP/wARP is a software suite for automated model building in X-ray crystallography. The [http://www.arp-warp.org ARP/wARP main page] has download instructions, a FAQ, and the User Guide. A...
• 12:5112:51, 10 February 2008 diff hist +234‎ m Modifying the protein to crystallize better ‎ →‎[[Lysine Methylation]]
• 12:5012:50, 10 February 2008 diff hist +216‎ m Modifying the protein to crystallize better ‎ →‎[[Lysine Methylation]]
• 12:5012:50, 10 February 2008 diff hist −217‎ m Lysine Methylation ‎No edit summary
• 12:4912:49, 10 February 2008 diff hist +3‎ m Modifying the protein to crystallize better ‎No edit summary
• 12:4912:49, 10 February 2008 diff hist −2‎ m Crystallization screens and methods ‎No edit summary
• 12:4812:48, 10 February 2008 diff hist +707‎ Expression and Purification: Tips and Tricks ‎No edit summary
• 12:4512:45, 10 February 2008 diff hist +1,055‎ N Crystallization screens and methods ‎ New page: * The PACT screen The PACT screen was developed by Janet Newman in the lab of Tassos Perrakis. This systematic screen, a pH-, anion- and cation-testing (PACT) screen, aims to decouple ...
• 12:2612:26, 10 February 2008 diff hist +838‎ N Lysine Methylation ‎ New page: This protocol is an NKI local adaptation based on the article by Grimes and co-workers[http://www.ncbi.nlm.nih.gov/pubmed/17098187?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_Re...
• 12:2412:24, 10 February 2008 diff hist +24‎ N Modifying the protein to crystallize better ‎ New page: * Lysine Methylation
• 12:2312:23, 10 February 2008 diff hist +50‎ m Topics ‎ →‎Crystal growth and manipulation
• 12:1512:15, 10 February 2008 diff hist +312‎ m Main Page ‎ a lits of chapter under "topics" is now added in front page, linked to the chapters
• 11:5811:58, 10 February 2008 diff hist +2,639‎ N Spheroplasts Plates ‎ New page: ==Day 0: Overnight growth of 96 selected clones== *Starting from rearraying list, perform an overnight culture of 96 clones in 1 ml of LB with antibiotics to taste. *To avoid any mistakes... current
• 11:5711:57, 10 February 2008 diff hist +2,062‎ N Spheroplasts Falcon ‎ New page: ==Spheroplasts lysis starting from Falcon tube cultures== *Prepare 50 ml falcon tubes with 12.5ml of medium plus antibiotics. *Transfer 125 uL of a saturated preculture. *Put falcons in i... current
• 11:5711:57, 10 February 2008 diff hist +221‎ N Expression screening ‎ New page: ==Expression Testing== *Cell lysis with Spheroplasts in 96-well Plates - From Darren Hart - Grenoble *[[Spheroplasts Falcon|Cell lysis with Spheroplasts in Falcon ... current
• 11:5311:53, 10 February 2008 diff hist +27‎ Topics ‎ →‎Expression and Purification
• 11:5211:52, 10 February 2008 diff hist 0‎ Topics ‎ →‎by package
• 11:5211:52, 10 February 2008 diff hist +15‎ Topics ‎ →‎by package