Crystal growth: Tips and Tricks: Difference between revisions

Crystal growth: Tips and Tricks (edit)

Revision as of 10:37, 15 February 2009

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→‎Reducing the mosaicity: links and rearrangement vs cryo articles

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 ==Improving crystals==
 ===Reducing the mosaicity===
-Things to try in the order crystallization-related / cryo-related / geometry-related:
-# change precipitant, pH, temperature; try additives
+Things to try concerning crystallization - see also [[Improving crystal quality]].
-# try co-crystallization with glycerol (2%, 5% or 10%).
+For cryo question see [[cryo]] and [[Data collection: Tips and Tricks]].
-# incubate your crystal longer in your cryo/stabilization solution.
-# optimize the cryo conditions
+* change precipitant, pH, temperature; try additives
-# anneal (freeze/thaw) the crystal - '''DISCLAIMER''': your mosaicity can go up as well as down with annealing
+* try co-crystallization with glycerol (2%, 5% or 10%).
-# the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis
+* incubate your crystal longer in your cryo/stabilization solution.
-# try smaller crystals (often growth faults lead to end of crystal growth)
+* optimize the cryo conditions
-# use a synchrotron beam (as it is less divergent than the usual home source, and the apparent mosaicity is the sum of the crystal mosaicity and the crossfire)
+* anneal (freeze/thaw) the crystal - '''DISCLAIMER''': your mosaicity can go up as well as down with annealing
-# shoot at parts of the crystal to find if there are good and bad parts (this requires a small beam)
+* the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis
-# look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??)
+* look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??)
 ==References==