Lysine Methylation: Difference between revisions

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Remove precipitated protein (up to 50%) by centrifugation
Remove precipitated protein (up to 50%) by centrifugation


Concentrate and put protein on Gel Filtration column in a Tris conatining buffer, eg:
Concentrate and put protein on Gel Filtration column in a Tris containing buffer, eg:


*20mM Tris (pH7.5)
*20mM Tris (pH7.5)
*200mM NaCl
*200mM NaCl
*1mM DTT
*1mM DTT
2

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