Incommensurate modulation

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In case of

  • unknown spacegroup and cell, AND
  • existence of reflection subsets that are offset from each other by a fractional displacement (not rotation!) in reciprocal space

you will find in IDXREF.LP a table like

***** RESULTS FROM LOCAL INDEXING OF    201 DIFFERENCE VECTOR CLUSTERS *****
MAXIMUM MAGNITUDE OF INDEX DIFFERENCES ALLOWED    8
MAXIMUM ALLOWED DEVIATION FROM INTEGRAL INDICES     0.050
MIMINUM QUALITY OF INDICES FOR EACH CLUSTER IN A SUBTREE    0.80
NUMBER OF CLUSTER SUBTREES      4
MERGE_TREE=     0.20
merged subtrees are marked by *
NODES              number of nodes belonging to subtree
DIFF_VEC           number of difference vectors in subtree
ORIGIN_INDICES     fractional indices of tree origin
ORIGIN_COORDINATES reciprocal space coordinates of tree origin
LENGTH             length of tree origin vector (1/A)
SUBTREE  NODES   DIFF_VEC   ORIGIN_INDICES        ORIGIN_COORDINATES         LENGTH
*  1      148     154546  0.00  0.00  0.00   0.000000  0.000000  0.000000  0.000000
   2       29      27949  0.13 -0.43  0.00   0.005978 -0.002899  0.000329  0.006652
   3       22      20055 -0.13  0.43  0.00  -0.005989  0.002904 -0.000323  0.006664

Inclusion of reflection subsets (subtrees) into the subsequent analysis of LATTICE BASIS (cell parameters and orientation) and CLUSTER INDICES is influenced by MERGE_TREE (default 0.2). That value can be set higher (e.g. 0.5) to merge less subtrees, or lower (e.g. 0.1) to merge more subtrees (the merged ones then also get a * in the first column above). Merging more subtrees will lead to longer cell parameters, and fractional CLUSTER INDICES.

Another indication of subtrees is in the table

***** RESULTS FROM LOCAL INDEXING OF   3000 OBSERVED SPOTS *****

 MAXIMUM MAGNITUDE OF INDEX DIFFERENCES ALLOWED    8
 MAXIMUM ALLOWED DEVIATION FROM INTEGRAL INDICES     0.050
 MIMINUM QUALITY OF INDICES FOR EACH SPOT IN A SUBTREE    0.80
 NUMBER OF SUBTREES     96

 SUBTREE    POPULATION

     1         1081
     2          846
     3          836
     4           29
     5           15
     6           12
     7           10
     8           10
     9            9
    10            8

which informs in this example that there are two subtrees of substantial size after the main subtree on which the subsequent indexing is based. However, that table also shows rotationally separated lattices!

In this example, we see a case of incommensurate modulation, with a vector 0.13 -0.43 0.00 and its opposite -0.13 0.43 0.00, meaning we have satellite reflections around each reflection of the main lattice. In reciprocal space, this looks like

view at reciprocal lattice of data from PDB 2WNQ obtained through https://zenodo.org/records/54568 using spot2pdb and coot through XDSGUI
view at reciprocal lattice of data from PDB 2WNQ obtained through https://zenodo.org/records/54568 using spot2pdb and coot through XDSGUI